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Wednesday, April 3, 2013

Timetable

DETERMINATION OF OSMOTIC CONCENTRATION IN POTATO (Solanum tuberosum) PARENCHYMA

By Dr. Elaine Winshell Updated and Revised by Dr. Susan Petro
Objectives: To understand that water moves across a cell membrane from a broad(prenominal)er immersion to a pull down one. To understand what is meant by hypotonic, hypertonic and isotonic resolves. To use using Excel to calculate the mean and standard deviation. To do linear regression graphing and using the graph-derived equations to determine the osmotic niggardness of the potato pargonnchyma (solving for x when you know y) Introduction: Living cells are bounded by selective membranes that allow certain substances in and exclude others. One substance that can easily queer a membrane is water. Water pass on move from high water potential (pure water) to lower water potential (solute change state in it). Therefore when a tissue is placed in an environment in which the solute submersion is lower and the water concentration is greater than that within the tissue, there will be a electronic network inward movement of water and the cells will swell. The declaration is called hypotonic.

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When a tissue is placed in a solution in which the solute concentration is higher and the water concentration is lower than that in the cells, water will move out of the cells and the cells will shrink. Such an environment is called hypertonic. When the water concentrations and solute concentrations on the outside and within are equal (isotonic), there will be no net movement of water. One way of determining the concentration of solute particles in the cell is to place the tissue in a variety of osmotic environments and determine which one causes no net uptake of water. Procedure Using a #5 secure borer, delete potato tuber cylinders from the potato. With a razor blade, cut the cylinders into lengths of exactly 40 mm. Be sure the cut is upright to the axis of the cylinder. Cut 24 cylinders. Label six 250 ml beakers as follows: 0.0, 0.1, 0.2, 0.3,...If you want to get a full essay, line of battle it on our website: Ordercustompaper.com



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