Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To sapidity the preparation of rent films from human blood sample. ii.To learn the techniques of discolor the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that grant in human blood sample under microscope. insertion: Leukocytes argon divided into granulocytes and agranulocytes. Granulocytes argon also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we bring back on forgather really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are overly pale, just about invisible and we will not see anything inside them. To be able to observe and reco gnize the disparate kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa chump and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene group blue (basic dye) and eosin ( vitriolic dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone message specimens.

The principle of Giemsa stain is the basic dye varnished the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to fall apart types of leukocytes. By patch o f the cells we can observe the different typ! es of snowy blood cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microscopes 10)Oil Immersion 11)Pasteur pipettes 12)Sorensens...If you want to get a full moon essay, fix it on our website: OrderCustomPaper.com

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