Evidence-Based Practice Paper and Intervention Presentation on Essay

Evidence-Based Practice Paper and Intervention Presentation on Diabetes (Benchmark Assessment) - Essay ExamplePrimarily, DM diagnosis relies on the measurement of plasma glucose levels. This diagnostic mode is efficacious in detecting diabetics and pre-diabetics, but it does not offer a clear mode of assessing resultant complications, which a great deal start in the vascular system. The diagnosis therefore, only tests tolerance to glucose, and not the associated risks such as the vascular risks (Pavri, 2001). The further assessment of risk may be necessary in monitoring the condition and judicatory of treatment, but it has not been available. However, a recent research by Zampetaki and others has led to the discovery of biomarkers that can be used in name and monitoring geek II diabetes and its associated risk (Zampetaki et al., 2010). Description of Diagnostic shaft The new diagnostic tool uses microarray screening and real-time polymerase chain reaction (PCR) processes to mon itor MicroRNAs (miRNAs), which have been positively associated with the development of the condition and its progression. This new tool offers significant prognostic ability to predict and monitor type II DM. The miRNAs are small group of RNAs not involved in coding. They instead work as repressors in translation (Zampetaki et al., 2010). The miRNAs bind to complementing sites by base pairing on untranslated portions of the target mRNA. They are prudent for directing repression or degradation of the transcripts. Research has shown that miRNAs influence stress responses, oncogenesis, development and angiogenesis. They also play a significant role in endothelial cell functioning as well as the statute of angiogenic potential and inflammations. The regard found that miRNAs change introductory to the start of type II DM (Zampetaki et al., 2010). The changes were narrow to miRNA-126, which was found to profoundly change with type II DM (Larkin, 2010). This provided proof for a plasma Micro-RNA jot for patients with type II DM, thus suggesting a possible prognostic value in monitoring and diagnosing DM (Zampetaki et al., 2010). Summary of Research The study on miRNAs and DM was part of a wider Italy-based Bruneck Research on various conditions including cardiovascular diseases. The study was initiated in 1990 by Kings College London British nerve center Foundation Centre (Larkin, 2010). The researchers sampled and measured miRNAs from a total of 822 participants. Some of the participants had type II DM, whereas others were controls. The measurements were conducted in tail fin phases on an annual basis from 1990 to 2005. The use of microarray screening and real-time PCR identified 700 miRNAs. The researchers then used miRNA relevance network inference technique to understand the miRNAs regulatory networks. The technique revealed changes in the levels of some five miRNAs prior to the start of type II DM. Notably, miRNA-126 was found to show a consistent sort o ut, which was correlated to the incidence and prevalence of type II DM (Zampetaki et al., 2010). The fact that miRNA-126 is significant in wound repair and vessel wellness implies that its decline is an indicator of vascular risk levels associated with DM. miRNA-126 is rich in normally functioning endothelial cells and its decline associated with DM may be an indicator of the progression of deterioration caused by DM, which increases vascular risk (Zampetaki et al., 2010). As such, miRNA can be a significant prognostic tool in monitoring DM and associated risks (Larkin, 2010). The study